pLO101 - Addgene
WEBPlasmid pLO101 from Dr. Berl Oakley's lab contains the insert ATET_07102 and is published in Fungal Genet Biol. 2018 Feb;111:1-6. doi: 10.1016/j.fgb.2018.01.003. Epub 2018 Jan 5. This plasmid is available through Addgene.
Defensive Function of Transposable Elements in Bacteria
WEBIn three different E. coli strains (S17, DH5α, and Nissle 1917), IS1 and IS10 rapidly disrupted the I-CeuI gene (encoding I-CeuI endonuclease) on the plasmid pLO11-ICeuI as early as
pLKO.1 puro - Addgene
WEBPlasmid pLKO.1 puro from Dr. Bob Weinberg's lab is published in RNA 2003 Apr;9(4):493-501. This plasmid is available through Addgene.
Genes Associated with the Proteins That Experienced the Highest …
WEBWhile an extensive BLAST analysis of 47 877 plasmids sequenced from the Addgene repository uncovered IS elements in only 1.12%, suggesting that IS contamination is not widespread, further tests...
Defensive Function of Transposable Elements in Bacteria
WEBAug 2, 2019 · IS1 and IS10 in three different E. coli strains (S17, DH5a and Nissle 1917) rapidly disrupted the I-CeuI gene (encoding I-CeuI endonuclease) on the plasmid pLO11-ICeuI as early as the 1st ...
pLKO.1 - TRC Cloning Vector - Addgene
WEBpLKO.1 is a replication-incompetent lentiviral vector chosen by the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via direct transfection, or can be converted into lentiviral particles for subsequent infection of a target cell line.
The Addgene repository: an international nonprofit plasmid and …
WEBJan 1, 2015 · Addgene Database queries start with the search box in the top right corner of every page at addgene.org. Scientists can search by gene name, gene alias, depositing scientist, publication author, Addgene Accession Number or any other relevant search term.
How do I search for a plasmid in your repository ... - Addgene …
WEBAddgene's website is designed to help you search our repository to find materials useful for your work. You can browse Addgene’s collection by using the search bar on the homepage or located in the upper right-hand section of our other website pages ( www.addgene.org ).
Pooled Library Amplifications - Addgene
WEBMay 30, 2024 · When a requesting scientist receives a pooled library from Addgene, they’ll need to amplify the sample for further downstream applications. As individual libraries can vary significantly, it’s important that we have an amplification protocol developed for each individual pooled library; generalized amplification protocols tend not to work ...
Addgene: Making Materials Sharing “Science As Usual” - PLOS
WEBNov 11, 2014 · Addgene-generated sequence data and depositor-provided sequence and validation data are made available online. The Addgene quality control process includes sequence validation before distribution to ensure that key features (insert junctions, mutations, etc.) in the plasmid record are accurate.
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