piRNA loading triggers MIWI translocation from the …
WEBMar 15, 2024 · By generating piRNA loading-deficient Miwi mutant mice, we demonstrate that loss of piRNA-loading ability in MIWI impedes its translocation from the IMC to CB during spermatogenesis. This ...
TDRD5 binds piRNA precursors and selectively enhances ... - Nature
WEBJan 9, 2018 · The pattern of piRNA deficiency in Tdrd5 cKO testes was similar to that of Miwi knockout (Miwi KO) mice, which exhibit normal MILI-piRNAs but devoid of MIWI-piRNAs 37,38 (Supplementary Fig....
Conditional inactivation of Miwi2 reveals that MIWI2 is only …
WEBJan 24, 2014 · The early meiotic arrest in Miwi2 global KO mice precludes further analyses of the potential roles of MIWI2 in late meiotic and haploid phases of spermatogenesis.
The Biogenesis and Function PIWI Proteins and piRNAs: Progress …
WEBConsistent with its expression pattern, miwi knockout mice exhibit male sterility, characterized by a block at the early spermatid stage. This phenotype is similar to defects observed in CREM (cAMP-responsive element modulator) deficient mice.
Mitochondrial membrane-based initial separation of MIWI and …
WEBMar 3, 2019 · In diverse species, different PIWI orthologs associate with distinct piRNA populations during different stages of germ cell development to fulfill specific functions. In mice, three PIWI proteins (MIWI, MILI and MIWI2) associate with two distinct developmental stage-specific piRNA populations .
Paternal pachytene piRNAs are not required for ... - Europe PMC
WEBDec 22, 2016 · Here, we report that Miwi KO round spermatids could fertilize both wild-type (WT) and Miwi KO oocytes through round spermatid injection, and could produce healthy and fertile offspring despite the global downregulation of …
Mili and Miwi target RNA repertoire reveals piRNA biogenesis and ...
WEBHITS-CLIP demonstrates that Miwi binds spermiogenic mRNAs directly, without utilizing piRNAs as guides, and independent biochemical analyses of testis mRNA-ribonucleoproteins (mRNPs) establishes that Miwi functions in the formation of mRNP complexes that stabilize mRNAs essential for spermiogenesis.
MIWI N-terminal RG motif promotes efficient pachytene piRNA
WEBNov 13, 2023 · To investigate the functional significance of RG motifs in mammalian PIWI proteins in vivo, we genetically engineered an arginine to lysine (RK) point mutation of a conserved N-terminal RG motif in MIWI in mice. We show that this tiny MIWI RG motif is indispensable for piRNA biogenesis and male fertility.
MIWI
WEBAug 14, 2018 · In mice, the nuclear Piwi protein MIWI 2 and Piwi‐interacting RNA s (pi RNA s) are required for DNA methylation of retrotransposon sequences and some other sequences. However, its underlying molecular mechanisms remain unclear.
Paternal pachytene piRNAs are not required for fertilization
WEBOur data indicate that aberrant MIWI-/MILI-bound pachytene piRNA profiles in spermatids do not affect fertilization, early embryonic development, or fertility of the offspring, suggesting that pachytene piRNAs might not be required for paternal transgenerational epigenetic inheritance in mice.