Turning on ARF: the Sec7 family of guanine-nucleotide-exchange …
WEBARF proteins are important regulators of membrane dynamics and protein transport within the eukaryotic cell. The Sec7 domain is ∼200 amino acids in size and stimulates guanine-nucleotide exchange on members of the ARF class of small GTPases. The members of one subclass of Sec7-domain proteins are direct targets of the secretion-inhibiting ...
Phylogenetic Analysis of Sec7-Domain–containing Arf Nucleotide ...
WEBJan 23, 2004 · Exchange is stimulated by members of another family of proteins characterized by a 200-amino acid Sec7 domain, which alone is sufficient to catalyze exchange on Arf. Here, we analyzed the phylogeny of Sec7-domain–containing proteins in seven model organisms, representing fungi, plants, and animals.
Regulating the large Sec7 ARF guanine nucleotide exchange …
WEBApr 13, 2014 · ARF GEFs are characterized by the presence of a conserved, catalytic Sec7 domain, though they also contain motifs or additional domains that confer specificity to localization and regulation of activity. These domains have been used to define and classify five different sub-families of ARF GEFs.
Four GTPases differentially regulate the Sec7 Arf-GEF to ... - PubMed
WEBSep 29, 2014 · The Golgi Arf-GEF Sec7 and the homologous BIG1/2 proteins are effectors of the Arf1 and Arl1 GTPases. We demonstrate that Sec7 is also an effector of two Rab GTPases, Ypt1 (Rab1) and Ypt31/32 (Rab11), signifying unprecedented signaling crosstalk between GTPase pathways.
Four GTPases Differentially Regulate the Sec7 Arf-GEF to Direct …
WEBSep 11, 2014 · The Golgi Arf-GEF Sec7 and the homologous BIG1/2 proteins are effectors of the Arf1 and Arl1 GTPases. We demonstrate that Sec7 is also an effector of two Rab GTPases, Ypt1 (Rab1) and Ypt31/32 (Rab11), signifying unprecedented signaling crosstalk between GTPase pathways.
Regulating the large Sec7 ARF guanine nucleotide exchange
WEBARF GEFs are characterized by the presence of a conserved, catalytic Sec7 domain, though they also contain motifs or additional domains that confer specificity to localization and regulation of activity. These domains have been used to define and classify five different sub-families of ARF GEFs.
Autoregulation of Sec7 Arf-GEF activity and localization by …
WEBOct 1, 2012 · Arf1 is activated at the TGN by Sec7, the founding member of the Sec7 family of guanine nucleotide exchange factors (GEFs) and a close homolog of the human ARFGEF2 implicated in congenital defects in cerebral cortex development.
Regulation of Arf Activation: the Sec7 Family of Guanine …
WEBAug 7, 2007 · The activation of Arfs is catalyzed by a family of guanine nucleotide exchange factors (GEFs), referred to as the Sec7 family, based on homology of their catalytic domains to the yeast Arf GEF, sec7p.
Structure of the Sec7 domain of the Arf exchange factor ARNO
WEBMar 5, 1998 · The structure of the Sec7 domain of ARNO (Arf nucleotide-binding-site opener) was solved by X-ray diffraction, using multiple isomorphous replacement and anomalous scattering, and refined to an...
The Sec7 Arf-GEF Is Recruited to the - Cell Press
WEBIn this study we have elucidated two key autoregulatory features of the TGN-localized Arf-GEF Sec7: autoinhibition and positive feedback. Both features require the function of a previously uncharacterized domain, the HDS1 domain.